Ocular Toxicology

Ocular Toxicology/Toxicokinetics

Using the most advanced ocular pharmacodynamics (PD), pharmacokinetic (PK) and toxicology evaluation techniques, the staff working in our dedicated laboratory help to advance drug development for a wide range of ophthalmic indications.

Our ophthalmologist-led services include:

  • Comparative ophthalmologic examination across a variety of toxicity studies, including eye irritation, new ocular drug toxicity and toxicokinetic (TK), PK and PD
  • Single- and repeat-dose ocular toxicology studies, conducted across a variety of species and via various dosage routes including systemic, topical, subconjunctival, intravitreal, sub-retinal and retrobulbar

Comparative Ophthalmology

For general toxicity studies, scientists on the comparative ophthalmology research team study the opthalmological toxicity of rodents, rabbits, canines, Non-human Primates (NHPs) and humans using slit-lamp and indirect ophthalmoscopy to examine the anterior and posterior segments of the eye.

Ocular Pharmacokinetics (PK)

Using state-of-the-art evaluation techniques, ocular PK studies assess tissue distribution and pharmacokinetics caused by ocular or systemic administration and can be conducted across a variety of species, tissues and liquids.

Eye Tissue Dissection

  • Conjunctivae
  • Cornea
  • Iris/ciliary body
  • Lens
  • Retina/choroid
  • Sclera
  • Optic nerve

 

Liquid Sampling

  • Aqueous humor
  • Vitreous humor
  • Serum

 

Evaluation Method

  • Enzyme-linked immunosorbent assay
  • Immunohistochemistry

 

Ocular Pharmacodynamics (PD)

Ocular PD studies assess the efficacy of new ocular drugs via more than 30 animal disease models, with additional services available to support cell- and gene-therapy drug development.

While new models can be developed to meet your specific needs, following are the main animal models available in-house:

 

  • Dry eye induced by benzalkonium instillation or by scopolamine injection
  • Allergic conjunctivitis induced by histamine instillation
  • Cataract induced by sodium selenite injection
  • Corneal neovascularization (CorNV) induced by stitching or by NaOH burn
  • Neurotrophic Keratitis (NK) induced by ciliary nerve damage
  • Acute glaucoma induced by intracameral injection of VISCOAT®
  • Chronic glaucoma induced by intracameral injection of magnetic microspheres
  • Chronic glaucoma induced by episcleral vein laser photocoagulation
  • Retinal ischemia reperfusion induced by elevating intraocular pressure
  • Optic nerve injury induced by clamping or partial transection
  • Choroidal Neovascularization (CNV) induced by laser photocoagulation
  • Retinal vascular leakage induced by AAV-VEGF injection
  • Central vein occlusion induced by laser photocoagulation
  • Oxygen-induced retinopathy (OIR)
  • Retinopathy induced by lighting
  • Retinitis pigmentosa NHP induced by sodium iodate injection